Animal manure is one of the most common sources of agricultural antibiotics that can adversely affect soil and water environment. Land application of animal manure can change the soil chemical properties, thus affecting fate and mobility of agricultural antibiotics in soil. The objective of this study was to determine the influence of soil chemical properties on the fate and transport of antibiotics in soil by conducting batch and column experiments. The soil chemical properties were pH (2.5-8.5), background electrolyte cation type (NaNO₃ and Ca(NO₃)₂), and dissolved organic carbon (DOC). Sulfamethazine (SMT) was used as a model compound in this study. Sorption of SMT by kaolinite and bentonite decreased as pH increased from 2.5 to 5 and slightly increased between pH 5 and 7. The results imply that sorption of SMT is mainly due to cation exchange at low pH and van der Waals interaction near neutral pH. Sulfamethazine exhibited a higher sorption affinity in the Na system than Ca system for both bentonite and two soils. Sorption of SMT to sandy loam surface soil was higher than to clay loam although sandy loam has low clay content. Subsoil having less organic content showed lower sorption affinity than surface soil. The results indicate that type and amount of soil organic matter play an important role in sorption of SMT and similar compounds. Sorption of SMT decreased in the presence of DOC extracted from dairy manure. Results from column experiments showed that retardation factor decreased with a presence of DOC in surface soil, while no difference in subsoil. One possible explanation is a strong affinity to DOC-SMT complex in subsoil that contains less organic matter.