Development of turf-type saltgrass cultivars is progressing at Colorado State University. Hybridization among ecotypes in greenhouses during the winter would facilitate cultivars development. Therefore, the influence of sampling time, nitrogen fertilization and burning on flowering induction of saltgrass ecotypes were evaluated over 2 years. In the first year 3 clones (A54 from Colorado, 49 from South Dakota and C66 from Nevada) were sampled from the field at 2 times. Clones 49 and C66 did not respond to the flowering induction treatments and were not included in statistical analysis. An analysis of flowering induction treatments of A54 indicated that sampling time, burning and nitrogen fertilization significantly influenced flowering (P<0.01). Burning doubled the number of flowers in plants dug and moved to the greenhouse in August without a significant effect on plants moved to the greenhouse in November. In the second year, 2 additional clones from Colorado (A18 and A61), and an additional sampling time in January were included. Clones C66 and A18 did not respond to flowering induction treatments and were not included in the statistical analysis. Clones, sampling time, nitrogen fertilization and burning had significant effects on flowering (P<0.01). Clone A54 had the greatest production of flowers. Overall, treatments obtained from the field in November produced 50% more flowers than those obtained in August, while January's treatments had 25% more flowers that November's. Sampling time had the best effect in A54 clone. Burning increased number of flowers in treatments from all 3 sampling time, but the increase was the greatest in August's. Nitrogen fertilization had greater effect on flowering in treatments that also received the burning treatment. Since saltgrass ecotype appears to be a major factor influencing flowering induction with the treatments used, further studies are necessary to evaluate flowering induction requirements of other ecotypes used in the breeding program.