Jia Yan, T. K. Danneberger, and David Gardner. The Ohio State University, The Ohio State University, Columbus, OH 43210
Light is the primary energy source for turfgrass growth and development. When light reaches grassed area under trees, both light intensity and quality are reduced dramatically. Low light level triggers series of detrimental morphological and physiological responses in turfgrass, including increased vertical shoot growth, decreased shoot density, root loss and so on. Gibberellin(GA) particularly increases excessive shoot growth and turf loss under low light conditions. Previous research showed that overexpression of GA 2-oxidase gene resulted in degradation of active GAs in plants, which led to dwarf, dark green phenotypes. The objective of this study was to find out whether transformation of GA2-oxidase gene would affect the shade response of creeping bentgrass(Agrostis palustris). Lines transformed with GA 2-oxidase gene and roundup ready gene, roundup ready lines, and non-transgenic cultivars ‘LS44' and ‘Crenshaw' were established in pots and field experiments under full sun, canopy shade, and artificial shade. Field and pot trials demonstrated that a range of shoot density, leaf color, leaf extension rate, and vertical shoot growth rate was observed within the transgenic lines. Some GA deficient lines showed constant dwarf phenotype and superior quality to other transgenic and control plants under all light conditions.
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