Sensitive to biotic and abiotic stresses and easy to be transformed make creeping bentgrass a good material for transgenic study in cool-season turfgrasses. However, as a cross-pollinated species, to confirm the effectiveness of a foreign gene in creeping bentgrass is difficult, especially for those quantitative traits, because each seed and the callus derived could have different genetic composition. To solve this problem, an efficient and right the genotype transformation system is needed. High quality embryogenic callus has been induced from nodes of one of our premium dollar spot resistant line. Using super binary Ti-plasmid as vector, hygromycin resistant gene as selection gene and uidA gene as maker gene, an efficient transformation system has been established. The entire process from initiation of embryogenic callus to production of transgenic plantlets was 70 - 85 days. The average transformation efficiency was 81%, or 200+14 differentiable transgenic lines per gram fresh callus. Compared with transgenic lines from calli derived from seeds, the morphology of transgenic lines from nodes showed highly homogeneous. The properties of high efficiency and right the genotype make this transformation system a good platform for transgenic study, biotechnological improvement and potential T-DNA tagging of target genes in creeping bentgrass.