Poultry litter can be used to fertilize sorghum-sudangrass pastures and hayfields. If properly managed, litter can be returned to land; however, one concern regarding land application of poultry litter is soil nutrient accumulation. Applying poultry litter to land at recommended crop N rates can lead to an accumulation of P, K, and micronutrients in the soil. This study was conducted from 2001-2004 to observe changes in characteristic soil fertility properties after fertilization with poultry litter and/or inorganic fertilizers. A completely randomized block design consisting of four treatments was utilized: an inorganic N, P, K fertilizer (I), poultry litter applied according to recommended P rate (PPL), poultry litter applied according to recommended N rate (NPL), and poultry litter applied at recommended P rate with supplemental inorganic N fertilizer (NPPL). In the poultry litter applications, plant available P was estimated to be 80% while N availability was estimated at 50% for the first year of the study. Soil samples from sudangrass hayfields were analyzed for pH, NH⁴⁺, NO^3-, Ca²⁺, Mg²⁺, PO₄^3-, K⁺, Cu²⁺, Fe³⁺, Mn²⁺, and Zn²⁺. Results from the four year study will be presented.