Waterlogging (WL) and poor soil aeration contributes to creeping bentgrass (Agrostis stolonifera L.) decline on golf greens. Oxygen deficiency is one of the primary root stresses in waterlogged soils. The objective of this study is to identify physiological traits in creeping bentgrass that are associated with depth of WL tolerance. Five cultivars of creeping bentgrass were subjected to WL at 10-, 50-, and 150-mm below the soil surface. No cultivar exhibited acceptable turf quality after 21 d constant WL at 10 mm below the soil surface. PennG-6 and L-93 had better quality than other three cultivars when WL occurred at 50 mm below soil surface. Penncross and Pennlinks had poor quality at all three depths of WL. Soil redox potential and chlorophyll content decreased with increasing depth of WL. Carbohydrate content and root metabolic activity will be further analyzed.