Louisiana sugarcane (Saccharum spp. hybrids) breeders are using Hawaiian (HI) genotypes to increase the genetic base of new cultivars. Cultivars developed in Hawaii have high tonnage potential, but are adapted to two-year crop cycles, while those developed in Louisiana accumulate high sucrose concentrations in a one-year crop cycle. Sugars and enzymes of sucrose metabolism were determined in internodes 2, 5, 8 and 11, from the top, of two Louisiana (LA) genotypes, HoCP 85-845 and LCP 85-384, and four HI genotypes, US 02-101, US 02-102, US 02-103, and US 02-104, grown in Schriever, Louisiana. Samples were taken during the grand growth and ripening stages in the plant and first ratoon crops. Total sugar concentration was significantly higher among fully-elongated internodes of the LA genotypes than among those of the HI genotypes, and a higher percentage of that sugar was sucrose. Only in US 02-103 did the sucrose concentration approach that in the two LA genotypes. Five enzymes were assayed: soluble acid invertase, neutral invertase, sucrose synthase, sucrose-phosphate synthase, and cell wall acid invertase. There were no consistent differences between genotypes in activity of any enzyme. Averaged across genotypes, the activity of sucrose synthase in elongating internode 2 was correlated with glucose and fructose concentration (r=0.72 and r=0.73, respectively). Activities of the invertases were not correlated with glucose or fructose concentrations. In internode 11, which was reaching maximum sugar accumulation, no enzyme was correlated with either total sugar or sucrose. The difference between sucrose synthesis and cleavage activities was also not correlated with sucrose concentration, as was suggested in other studies of highly diverse sugarcane genotypes. The LA and HI genotypes were clearly different in sugar accumulation, but these differences could not be explained by activities of sucrose metabolism enzymes in crude extracts.