High grain protein concentration (GPC) is an essential trait for bread wheat cultivars, and producers receive financial incentives for hard red spring grain with GPC equal to or exceeding 14%. A chromosomal region from Triticum turgidum ssp. dicoccoides, reported to confer a 1 to 2% increase in GPC, was recently transferred to hexaploid wheat. The objective of this research was to assess nitrogen response differences among backcross derivatives of Scarlet and Tara 2002 with and without the high GPC region at varying nitrogen levels. This study was conducted in a direct seeded field trial in Dusty, WA in 2004. Four replicates of each of eight genotypes were evaluated with 0, 45, 90, 135, and 180 kg N/ha, 45 kg N/ha of which was applied the previous fall. The remainder was deep-banded below the seed at planting. Two, 90-cm rows of plant material were hand harvested at physiological maturity. The above ground biomass and grain were analyzed for nitrogen and sulfur content, and partitioning among grain and stover fractions was determined. Nitrogen rate significantly impacted every variable tested including grain yield and GPC. GPC of >14% were achieved when grain yields plateaued demonstrating that yield potential must be satisfied before grain protein can be elevated to desirable levels. Tara 2002 produced more grain than Scarlet and its derivatives, particularly at low N rates; however, derivatives with the high-GPC region were not distinguishable from Tara 2002 or Scarlet for grain yield or for GPC at any fertility rate. Test weights of grain harvested from all entries were extremely low suggesting that plants were subjected to high levels of abiotic stress, which may have influenced these results. This field trial will be repeated in 2005.