Corn (Zea Mays L.) is the main crop in the United States and starch is the most important derived product from the corn kernels. This study was conducted to determine whether Corn Belt lines introgressed with exotic materials from Argentina, Chile, Uruguay, Cuba and Florida have appropriate wet milling characteristics. Ten lines from the Germplasm Enhancement of Maize project with different starch content were analyzed using both the Near-Infrared Reflectance (NIR) technology and the 100-g wet-milling procedure. The wet milling efficiency of exotic corn lines was correlated positively with starch content. Statistical differences (Alpha=0.05) were found for yield of the wet milling fractions (starch, fiber, gluten, and germ). Residual protein levels in the starch and gluten fractions were in the range of 0.26-0.32% and 38-45%, respectively. Oil content in the germ varied from 50 to 60%. Our results indicate that the use of exotic corn germplasm in a wet milling breeding program will enhance available genetic diversity.