Stripe rust (yellow rust) caused by Puccinia striiformis Westend. f.sp. tritici Eriks., is one of the most serious leaf diseases of wheat in the world. Although numerous disease resistance genes have been isolated in dicots recently, only a few have been reported in monocots. The wheat cultivar Moro is resistant to the prevalent stripe rust races in western Canada. The stripe rust resistance locus Yr10 is located on chromosome 1B in Moro and originates from the Turkish line PI 178383. Two full length NBS-LRR genomic sequences 4B and 4E have been isolated. Based on RT-PCR and promoter analysis results, sequence 4B appears to be expressed while 4E is not. The expressed NBS-LRR sequence had a perfect linkage in segregating populations totalling 874 lines. A full-length cDNA sequence of 2.5kb was isolated by RT-PCR and this cDNA sequence was identical to the sequence of genomic clone 4B with the exception of two nucleotides. The genomic clone 4B was introduced in the susceptible wheat cultivar Fielder using an improved regeneration protocol for monocots and resistant plants were obtained demonstrating that the sequence encoded by clone 4B is the Yr10 resistance gene.