Soybean is the second most important crop in the Delmarva region of the United States. The agronomic traits of eight soybean breeding lines were evaluated at the Agricultural Experiment Research Station, University of Maryland Eastern Shore, Princess Anne, MD in 2003 and 2004 growing seasons. The breeding lines were compared with four commercial varieties of soybean such as Hutcheson, Stalwart, Charleston and Croton. The variables evaluated included seed yield, biological yield and heights of plants. The highest seed yield was given by breeding lines D38B3009 and M3583009 which were similar to Stalwart. However, breeding lines E3582019, D6874003 and D6872615 gave lower seed yield. Breeding line D6872031 gave higher biological yield which was similar to Stalwart and Hutcheson. There were also significant differences in the heights of plants. Breeding lines E3563010, D6874003 and Croton had the maximum heights. Some of the breeding lines which provided higher or statistically similar seed yield to commercial varieties could play an important role to increase the yield of soybean in the Delmarva region.