Powdery mildew of wheat, caused by Blumeria graminis f.sp. tritici, is a prevalent fungal disease in the southeastern United States. The use of host resistance as a method to control the disease and prevent yield loss requires plant breeders to constantly search for new resistance sources. Inheritance of powdery mildew resistance was studied in four populations derived from crosses between the NCSU germplasm lines NC96BGTD3, NC96BGTA5, NC96BGTA6, NC97BGTD7 and the susceptible cultivar Saluda. Results from phenotypic evaluations of F2-derived lines in greenhouse and field tests showed that powdery mildew resistance exhibited a 1 resistant: 2 segregating: 1 susceptible segregation ratio consistent with a monogenic trait in all four populations. Microsatellite markers linked to resistant genes were found in all four populations. The gene in NC97BGTD7 is postulated as a new powdery mildew resistance gene located on chromosome 5DL. Field evaluations of three populations derived from the crosses between resistant germplasm lines, NC96BGTD3 x NC97BGTD7, NC96BGTA6 x NC97BGTD7, and NC96BGTA5x NC99BGTAG11 indicated that two independently segregating powdery mildew resistance genes were present in each of the populations.