The oil content of flaxseed has been determined on entries in the Flax Uniform Regional Nursery (FURN) since 1940. Locations in the FURN were represented in latitude at Pesgah Forest, NC in the south and Saskatoon, Sask in the north. The ranges in longitude were Ottawa, Ontario in the east and Corvallis, Or in the west. In an attempt to evaluate the effect of latitude and longitude on oil content of flaxseed, the 60 years of data from the FURN was used to estimate the effect of latitude and longitude of the production location on the oil content of the seed produced. Selected data from the present flax growing area (adjusted for a wide range of genotypes) would suggest an increase in oil content from south to north. The effect of latitude was very small. In an attempt to verify the initial observations from the regional tests, follow up studies are underway to evaluate oil content from various locations in NE, SD, ND, Morden and Saskatoon. All locations will produce seed of the same cultivars and all the samples will be processed in a common laboratory to minimize the possible effect of different cultivars and methods of determination of oil content.