As part of an effort to expand the genetic base of Upland cotton, we are introgressing germplasm from two wild species, Gossypium tomentosum and G. mustelinum, into Upland cotton (G. hirsutum). As part of this effort, we are testing the efficacy of random-mating populations for interspecific breeding. Here we report on the results of the performance of multiple generations of interspecific populations in College Station during 2003 and 2004, the effects of random-mating on means and variances, as well as individuals that show promise for selection. Generations available included: TM-1, the Ms4 line, the wild species, F1, BC1F1 to TM-1 and Ms4, BC1rm1, BC1rm2, BC1F2 and two commercial checks: FM832 and PSC355. Fiber quality parameters, plant characteristics, and molecular marker analysis to test effects on map distances as well as retention of alien alleles will also be discussed.